Q.2: The CAP site functions as a spacer to separate the inhibitor from the promoter a structural gene an inhancer for the attachement of RNA polymerase inhibtor of the Plac site Q.3 : At high glucose levels and high lactose levels the structural genes are not transcribed because the repessor protein attaches to the operator preventing gene structural transcription the CAP protein can not bind to the CAP site resulting in no enhancement the CAP protein binds to the CAP site preventing gene transcription the lactose / repressor protein complex binds to the operator preventing gene transcription Q.4 : The TATA box is a highly preserved DNA sequence in eukaryotic genes is near the promoter region of eukaryotic genes plays an imortant role in the attachment of RNA polymerase all of the above none of the above Q.5 : In gel electrophoresis DNA migrates to the anode because it is positively charged. larger DNA fragment moves faster because they have more negative charges DNA migrates to the cathode because it is negatively charged DNA migration speed is directly proportional to the size of the fragment none of the above Q.6. A genomic library is the genomic DNA presents a particular gene of an organism are gel electrophoretic bands of DNA fragments obtained through restriction enzyme cleavage is a collection of foreign DNA fragments inserted into a cloning vector Q.7 : In order for PCR to be successful, it is necessary to have large quantities of DNA to start with. have a short piece of RNA that has its complementary base sequence on the DNA to be amplified know the base sequence of the DNA to be amplified have viral or plasmid DNA Q.8: Which statement does not characterize "Transposons" DNA sections that can move from one gene location to another can move only when excised by specific restriction enzymes can cause a mutation in a gene all of the above are correct Q.9 : Which statement about transcription in eukaryotes is false? a DNA sequence, which is very distant from a gene may have an influence on its transcription without the proper transcription factors no mRNA is produced after transcription a mRNA remains unaltered and is directly used in translation not all transcriptions produce mRNA Q.10 : Which is not an interaction between DNA and protein domain Zn finger Leu zipper Helix turn helix Homeo domain all of the above are interactive domains Q.11 : A second screening step is often necessary to find bacteria that have taken up plasmids with foreign DNA inserts. Which statement is true about the second screening step it eliminates bacteria that have no plasmids at all. bacteria having plasmids with foreign DNA inserts will not grow on the cultural medium bacteria having plasmids with foreign DNA inserts will grow on the cultural medium only bacteria without plasmids will grow on the cultural medium Q.12 : Which statement about telomerases is not correct ? they help making a complete complementary copy of a DNA strand. in absence of telomerases the 5' end of the replicated DNA is not complete cancer cells are shown to have active telomerases all are correct Q.13: Which statement about stem cells is false are undifferentiated cells can only be harvested from embryos in their earliest developmental state can develop in vitro into speciallized cells using differentiating media all are correct Q.14 Which protocol describes best the cloning of animal. a stem cells from a donor is implated in the uterus of the surrogate egg is removed from donor, placed in special growth media, egg cells are implanted in uterus of surrogate egg (nucleus removed) is fused with donor cell, placed in cult. media. Cell implanted in uterus of surrogate donor DNA isolated, injected into egg (nucleus removed), egg implated in uterus of surrogate Q.15 : What property of a restriction enzyme allows DNA from different sources to be joined they restrict certain DNA sequences from being transcribed they cut preferentially plasmid DNA if the same restriction enzyme cuts two different DNAs, the ends of the fragments are complementary they cut ranomly at sites within the DNA all answers are false