1. Which statement about telomeres is false

a.   Telomeres are  moderately repetitive DNA segments at the ends of  chromosomes. b.   The 5' end of the replicated strand is not completely replicated

c.  Cells that do not have telomerase have chromosomes that lose 50 to 200

     bp of telomeric DNA with each cell division.

d. Telomerase is found in cells that constantly divide, including most cancer cells.

e. all are correct

 

2. What type of structural interactions are possible between DNA and protein

 

3. What are the components of an operon

 

4. Consider the lac l gene in the lactose operon. What would happen to  the structural gene lac Z if the TATA box  was deleted from the lac I promoter?

a. The lac Z gene would never be transcribed

b. The repressor protein would be made normally, but it would not bind to the operator

c. The repressor protein would not be made and lac Z expression would be possible

 d. The lac I repressor protein would accumulate quickly

 

5. Which statement is false

a. Some types of transposons involve an RNA copy that serves as a template for new DNA.

b.  Some types of  transposons are transcribed, but never translated.

c. The genetic organization of some transposons resembles that of retroviruses.

d. Transposons may have played a role in the formation of new proteins.

e. the alu family of transposon have promoter regions that are sensitized by hormones

f. Transposon activity is restricted to meiosis

 

6. Three DNA samples ( 1 kbp,  2kbp 3 kbp ) are separated by gel electrophoresis. Which statement is false

a. the 1 kbp sample runs twice as fast as the 2 kbp sample

b. the elution order is 3 kbp (slowest),  2kbp,  1kbp (fastest)

c. al samples migrate toward the anode

d. all statements are correct

 

7. Which is not a requirement for PCR

a. RNA primer

b. plasmid DNA

c. temperature stable DNA polymerase

d. dNTPs

e all are required

 

8. Which statement about RNA splicing is not true?

   a. It removes introns.

   b. always removes the same introns.

   c. It is a post transcriptional process .

   d. It shortens the RNA molecule.

 

9. Which statement about transcription factors in eukaryotes is false

   a. bind to DNA, and then RNA polymerase can bind.

   b. need to be present before transcription can begin

   c. will interact with any gene

   d. are always bound to DNA.

   e. may have helix-loop-helix domains for DNA binding and recognition.

 

10. Which of the following are not used in transcriptional regulation in eukaryotes?

   a. Enhancers

   b. Transcription factors

   c. RNA polymerase subunits

   d. Promoters

   e. repressors

   f. all are part of transcriptional regulation in eukaryotes

 

11. The  pBR322 plasmid, which is used as a cloning vector has an amp – gene (coding for ampicillin resistance and a Tc – gene coding for tetracycline resistance. Explain why both genes are necessary for recombinant DNA  experiments

 

12. Explain the process by which active genes (genes that are expressed) can be monitored

 

13. Genetic engineering has made large scale production of insulin possible. Describe the individual steps in this type of insulin production.

 

14. How would you carry out a PCR experiment

 

15. What are VNTR and how are they used in forensic analysis